Центральная Научная Библиотека УрО РАН

Вид документа : Статья из журнала
Шифр издания : 57/I-56
Автор(ы) : Deryabin D. G., Sviridova T. G., El'-Registan G. I., Chereshnev V. A.
Заглавие : Impact of bacterial autoregulatory molecules (homoserine lactones and alkylhydroxybenzenes) on the oxidative metabolism of the cell effectors of natural immunity
Место публикации : Microbiology. - 2013. - Vol. 82, № 2. - С. 133-141
ББК : 57
Предметные рубрики: БИОЛОГИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): alkylhydroxybenzenes--chemoluminescence--homoserine lactones
Аннотация: We investigated the impact of bacterial regulators homoserine lactones (HSLs) and alkylhydroxybenzenes (AHBs) (which are present in human fluids at pico- and nanomolar concentrations) on neutrophile oxidative metabolism. The HSL and AHB effects were determined using a test based on induced luminol-dependent chemoluminescence of neutrophiles in human peripheral blood. In this test, neutrophiles were preincubated with chemical analogs of bacterial autoregulators with different lengths of the hydrocarbon radical, such as HSL · HCl, C6- and C12-HSL, and C1-, C6-, and C12-AHB. We revealed that they suppressed the chemoluminescence and, accordingly, the oxidative metabolism of neutrophiles. This effect was more significant with HSLs than with AHBs. Within each of the two groups, the effect increased with an increase in the length of the hydrocarbon chain of the homologues. High concentrations of long-chain autoregulators of both types produce a cytotoxic effect that is associated with apoptosis in the case of C12-HSL and with cell membrane damage in the case of C12-AHB. The effects of low HSL and AHB concentrations involve their protein-modifying properties and result in changes in the activities of neutrophile oxidative enzymes. To a lesser extent, these effects are due to the pro- and antioxidant activities of HSLs and AHBs, respectively. In light of the results obtained, the HSL and AHB effects are to be considered as a novel mechanism of regulating the activities of cell effectors of natural innate immunity. In symbiotic and parasitic systems, the mechanism involves the bimodal pattern of the effects of HSLs and AHBs that vary depending on their structure and concentrations
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Вид документа : Статья из журнала
Шифр издания : 57/A 41
Автор(ы) : Chereshnev V. A., Rodionov S. Yu., Vasilyev N. V., Orlov O. A., Cherkassov V. A.
Заглавие : Alpha-fetoprotein immunotherapy as a stage of combined treatment of cancer patients
Место публикации : Voprosy Onkologii . - 2005. - Vol.51, №1. - С. 86-92
ББК : 57
Предметные рубрики: БИОЛОГИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): alpha fetoprotein--antibody--apoptosis
Аннотация: The paper discusses the preliminary findings on alpha-feto-protein (AFP) treatment of 58 patients with stage III-IV malignancies of different localizations.influence on neoplastic processes was registered in poorly differentiated cell tumors, nor were any immunological mechanisms of antitumor action triggered on. However, in moderately- and well-differentiated cell tumors, several foci of acute immune inflammation were induced. It is suggested that AFP is responsible for tumor cell apoptosis by freeing antigenic determinants from shielding antibodies via elimination of «immunological enhancement» of tumor growth.
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Вид документа : Статья из журнала
Шифр издания : 61/T 10
Автор(ы) : Saidakova E. V., Korolevskaya L. B., Shmagel K. V., Shmagel N. G., Chereshnev V. A.
Заглавие : T cell apoptosis in HIV-infected patients with incomplete immune recovery after antiretroviral therapy
Место публикации : Doklady Biological Sciences. - 2013. - Vol. 450, № 1. - С. 189-191
Примечания : Библиогр.: с. 191 (5 ref.)
ББК : 61
Предметные рубрики: ЗДРАВООХРАНЕНИЕ. МЕДИЦИНСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): antiretroviral therapy--hiv-infected --t cell
\\\\expert2\\nbo\\Doklady Biological Sciences\\2013. V. 450, N 1. P. 189-191.pdf
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Вид документа : Статья из журнала
Шифр издания : 61/C 98
Автор(ы) : Sviridova T. G., Deryabin D. G., Cyganok O., Chereshnev V. A.
Заглавие : Cytotoxicity of N-dodecanoyl-L-homoserine lactone and 5-N-dodecyl resorcinol to human granulocytes and monocytes: A comparative study
Место публикации : Central European Journal of Immunology. - 2013. - Vol. 38, № 3. - С. 310-316
Примечания : Bibliogr. : p. 316 (21 ref.)
ББК : 61
Предметные рубрики: ЗДРАВООХРАНЕНИЕ. МЕДИЦИНСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): 5-n-dodecyl resorcinol--cytotoxicity--apoptosis
Аннотация: Small molecules originating from microbes (SMOMs) play a significant role in bacterial-bacterial communication and in interactions between bacteria and their hosts. The aim of this study is a comparative analysis of the cytotoxic activity to human granulocytes and monocytes of two structurally close SMOMs: N-dodecanoyl-L-homoserine lactone (C12-HSL) and 5-N-dodecyl resorcinol (C12-AR). Cell viability was determined by Trypan blue dye exclusion. To distinguish cell death mechanisms, both necrosis and apoptosis tests were carried out. Cells undergoing apoptosis were identified by caspase-3 activity and the level of histone-associated DNA fragments. To evaluate cell lysis, the lactate dehydrogenase release test was used. In addition, the SMOM's action on erythrocyte membrane stability was investigated. The investigated SMOMs in micromolar concentrations showed more dose-dependent cytotoxicity against granulocytes than monocytes, but they used different mechanisms to impinge on the cell death pathway. C12-HSL specifically induced apoptosis similar to the activity previously reported for 3-oxo-N-dodecanoyl-L- homoserine lactone that originated from Pseudomonas aeruginosa. In contrast, C12-AR induced fast cytolytic effects (necrosis) as shown by the release of cytoplasmic lactate dehydrogenase, and presumably were defined by cellular membrane destabilisation. Our data demonstrate that both C 12-HSL and C12-AR can eliminate key defence cells, which would otherwise participate in the destruction of pathogenic bacteria. These results reinforce the SMOMs bifunctionality concept that such bacterial molecules not only regulate bacterial-bacterial interactions but also break immune defences as a new and important mechanism of host evasion
\\\\expert2\\nbo\\Central European Journal of Immunology\\2013. Vol.38, №3. С. 310-316.pdf
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Черешнев В. А., Оникиенко С. Б., Земляной А. В., Абкин С. В.
Заглавие : Применение защитных белков теплового шока-70 для борьбы с COVID-19
Место публикации : Журнал инфектологии. - 2022. - Т. 14, № 2, Приложение 1: Материалы VIII Конгресса Евро-Азиатского общества по инфекционным болезням, Санкт-Петербург, 17-19 мая 2022. - С. 134-135
Предметные рубрики: ЗДРАВООХРАНЕНИЕ. МЕДИЦИНСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): covid 19--коронавирус--шок тепловой--белки защитные
Аннотация: We investigated the impact of bacterial regulators homoserine lactones (HSLs) and alkylhydroxybenzenes (AHBs) (which are present in human fluids at pico- and nanomolar concentrations) on neutrophile oxidative metabolism. The HSL and AHB effects were determined using a test based on induced luminol-dependent chemoluminescence of neutrophiles in human peripheral blood. In this test, neutrophiles were preincubated with chemical analogs of bacterial autoregulators with different lengths of the hydrocarbon radical, such as HSL · HCl, C6- and C12-HSL, and C1-, C6-, and C12-AHB. We revealed that they suppressed the chemoluminescence and, accordingly, the oxidative metabolism of neutrophiles. This effect was more significant with HSLs than with AHBs. Within each of the two groups, the effect increased with an increase in the length of the hydrocarbon chain of the homologues. High concentrations of long-chain autoregulators of both types produce a cytotoxic effect that is associated with apoptosis in the case of C12-HSL and with cell membrane damage in the case of C12-AHB. The effects of low HSL and AHB concentrations involve their protein-modifying properties and result in changes in the activities of neutrophile oxidative enzymes. To a lesser extent, these effects are due to the pro- and antioxidant activities of HSLs and AHBs, respectively. In light of the results obtained, the HSL and AHB effects are to be considered as a novel mechanism of regulating the activities of cell effectors of natural innate immunity. In symbiotic and parasitic systems, the mechanism involves the bimodal pattern of the effects of HSLs and AHBs that vary depending on their structure and concentrations
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Сиглы отделов ЦНБ УрО РАН

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  бф - Научно-библиографический отдел

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  ИИиА -Фонд исторической литературы в ЦНБ УрО РАН

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  кх - Отдел фондов (книгохранениe)

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  орф - Обменно-резервный фонд

  пф - Читальный зал деловой и патентной информации

  рк - Фонд редкой книги

  ч/з - Главный читальный зал

  эр - Зал электронных ресурсов

Сиглы библиотек институтов и НЦ УрО РАН

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