P 93 Proliferativeresponse of lymphocyte to pokeweed mitogen depends on the concentration of endogenous cortisol in the early post-traumatic period in patients with penetrating eye injury / V. A. Chereshnev, Yu. I. Shilov , T. V. Gavrilova [et al.] // Bulletin of experimental biology and medicine. - 2012. - Vol. 153, № 5. - P722-725. - Bibliogr. : p. 724-725 (15 ref.) Рубрики: БИОЛОГИЧЕСКИЕ НАУКИ Кл.слова (ненормированные): LYMPHOCYTES -- CORTISOL -- PENETRATING EYE INJURY Аннотация: The intensity of lymphocyte proliferation in response to pokeweed mitogen depends on cortisol level in the peripheral blood in the early post-traumatic period of penetrating eye injury. Lymphocyte proliferation in 72- and 96-h cultures from patients with high levels of endo genous hormone was suppressed. In 120-h cultures, the intensity of proliferation remains unchanged. Lymphocyte blast transformation was increased in 120-h cultures from patients with normal cortisol concentration and remained unchanged in case of low cortisol level \\\\expert2\\NBO\\Bulletin of Experimental Biology and Medicine\\2012, v.153, N 5, p. 722-725.pdf |
D 44 Dependence of the lymphocyte proliferative response on the endogenous cortisol level and sensitivity to β-adrenergic regulation in vitro in the early period of penetrating eye injury / V. A. Chereshnev, M. V. Chereshneva, Yu. I. Shilov, T. V. Gavrilova // Doklady Biological Sciences. - 2010. - Vol. 434, № 1. - P304-306. - Bibliogr. : p. 306 (10 ref.) Рубрики: ЗДРАВООХРАНЕНИЕ. МЕДИЦИНСКИЕ НАУКИ Кл.слова (ненормированные): CELL PROLIFERATION -- DRUG EFFECT -- EYE INJURY \\\\expert2\\NBO\\Doklady Biological Sciences\\2010. V. 434, N 1.P. 304-306.pdf |
Z 86 Zotova, N. V. Systemic inflammation: methodological approaches to identification of the common pathological process / N. V. Zotova, V. A. Chereshnev, E. Yu. Gusev // PLOS One. - 2016. - Vol. 11, № 5. - P. e0155138 Рубрики: ЗДРАВООХРАНЕНИЕ. МЕДИЦИНСКИЕ НАУКИ Кл.слова (ненормированные): MULTI-SYNDROME -- PATHOLOGICAL PROCESS Аннотация: We defined Systemic inflammation (SI) as a “typical, multi-syndrome, phase-specific pathological process, developing from systemic damage and characterized by the total inflammatory reactivity of endotheliocytes, plasma and blood cell factors, connective tissue and, at the final stage, by microcirculatory disorders in vital organs and tissues.” The goal of the work: to determine methodological approaches and particular methodical solutions for the problem of identification of SI as a common pathological process. SI can be defined by the presence in plasma of systemic proinflammatory cell stress products—cytokines and other inflammatory mediators, and also by the complexity of other processes signs. We have developed 2 scales: 1) The Reactivity Level scale (RL)–from 0 to 5 points: 0-normal level; RL-5 confirms systemic nature of inflammatory mediator release, and RL- 2–4 defines different degrees of event probability. 2) The SI scale, considering additional criteria along with RL, addresses more integral criteria of SI: the presence of ≥ 5 points according to the SI scale proves the high probability of SI developing. To calculate the RL scale, concentrations of 4 cytokines (IL-6, IL-8, IL-10, TNF-α) and C-reactive protein in plasma were examined. Additional criteria of the SI scale were the following: D-dimers>500ng/ml, cortisol>1380 or <100nmol/l, troponin I≥0.2ng/ml and/or myoglobin≥800ng/ml. 422 patients were included in the study with different septic (n-207) and aseptic (n-215) pathologies. In 190 cases (of 422) there were signs of SI (lethality 38.4%, n-73). In only 5 of 78 cases, lethality was not confirmed by the presence of SI. SI was registered in 100% of cases with septic shock (n-31). There were not significant differences between AU-ROC of CR, SI scale and SOFA to predict death in patients with sepsis and trauma. \\\\expert2\\NBO\\PLoS ONE\\2016, v.11, p. e0155138pdf.pdf |