AIM: To establish a one-stage model of experimental acute necrotizing pancreatitis (ANP) in rats characterized by the simplicity of performance and high degree of repeatability. METHODS: ANP modeling in rats was performed based on modification of the ligation model as follows: synthetic material ligature using an atraumatic needle was performed to capture pancreatic gland ducts and marginal duodenum vessels. Ligature tips were exteriorized to the abdominal wall, and the ligature was skinned over to avoid catching intestine loops. Pancreatic macroscopic appearance and histological changes were observed. Blood biochemical and hemostatic indicators were also determined. RESULTS: Laboratory analysis of rats with experimental ANP showed a pattern of disturbances similar to that observed during pancreatic necrosis in humans as soon as the first day. General blood analysis revealed enhanced leukocytosis and alterations in leukogram characteristics, indicating acute inflammation...